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To develop an in-house nucleofection buffer for RAW 264.7 cells, we first electroporated the cells with pGL3 luciferase reporter plasmid with different compositions. Among the solutions tested, Sol I showed the highest transfection efficiency in RAW 264.7 cells ( …

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A mill is a device that breaks solid materials into smaller pieces by grinding, crushing, or cutting. Such comminution is an important unit operation in many processes.There are many different types of mills and many types of materials processed in them. Historically mills were powered by hand (e.g., via a hand crank), working animal (e.g., horse mill), …

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To maintain an assurance of raw material supply, suppliers must have sufficient production capacity to produce a given buffer component reliably in accordance with specifications defined by appropriate quality unit(s), in needed quantities, and within required time frames.

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